R1780

Sigma

 

RPMI-1640 Medium

With L-glutamine and sodium bicarbonate. Without arginine, leucine, lysine, and phenol red, liquid, sterile-filtered, cell culture tested

MDL number:MFCD00217820

Related Products

References

Reviews

Description

ApplicationRPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy's 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.
ReconstitutionDesigned for use in stable isotope labeling with amino acids in cell culture (SILAC) applications.
Supplement with 0.100 g/L L-Arginine-13C6, 15N4 HCl (Product 608033), 0.100 g/L L-Leucine-13C6, 15N (Product 608068), and 0.100 g/L L-Lysine-13C6, 15N2 (Product 608041).

Properties

sterilitysterile-filtered
formliquid
total impurities Endotoxin, tested
suitabilitycell culture tested